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Department of Biological Sciences
Molecular Neuroscience
Developmental Biology
Cellular Genetics
Molecular Genetics
Plant Development and Physiology
Cellular Biochemistry
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Plant Environmental Responses
Environmental Microbiology
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Photosynthetic Microbial Consortia
Photo Photosynthetic Microbial Consortia Laboratory
The laboratory has started on October 1st, 2015 as a donated laboratory from Institute for Fermentation, Osaka.
New graduate students are welcome to apply for April 2016 admission.

This laboratory consists of:
Vera [Omics witch] Thiel, associate professor
Marcus [Smart bug isolator] Tank, associate professor
Sakiko [Genetic artist] Nagashima, research assistant professor
Nanako [Metabolomics expert] Kanno, project researcher
Setsuko [Beloved by APB] Hirose, project researcher
Shunichi [Match] Fukushima, project researcher
Yuki [Hyper chatterbox] Takabe, JSPS Research fellow
Keizo [Walking dictionary] Shimada, visiting professor
Satoshi [Great stupid] Hanada, professor
Prof Satoshi Hanada e-mail
Asc Prof Vera Thiel e-mail
Asc Prof Marcus Tank e-mail
Taxonomy and environmental microbiology of photosynthetic prokaryotes in nature
Microbial consortia majorly consisted of photosynthetic prokaryotes are frequently found in various aqueous environments in the world. The organisms that can grow by photosynthesis play an important role not only in the supply of energy from light to the consortia but also in the formation and maintenance of microbial mats or films of the consortia. However, it has not been clear how the photosynthetic prokaryotes contribute to the formation and maintenance of these consortia. The aims of this laboratory are to reveal the formation mechanism of the photosynthetic microbial consortia and the contribution of photosynthetic prokaryotes to the stabilization of them. Also, we are aiming to control the photosynthetic microbial consortia and to make use of them for the solution of energy and environmental problems.
The formation of microbial mats in hot springs
In neutral and slight alkaline hot springs, distinctive microbial consortia called microbial mats are often observed. The microbial mats often contain filamentous anoxygenic phototrophic bacteria, Chloroflexus species. These bacteria are able to move by gliding and the motility has possibly a close relation to the formation and maintenance of the microbial mats. However, it remains unsolved how the microbial mats are formed and maintained. In addition, the gliding mechanism of Chloroflexus species is not clear yet. One of our purposes is to elucidate the unknown mechanisms of the gliding motility and the effect of the motility in the mat formation.
The taxonomic rearrangement of thermophilic cyanobacteria
Various thermophilic cyanobacteria are commonly observed in the microbial mats of hot springs in addition to Chloroflexus species. These oxygenic phototrophs supply the energy from light to many other inhabitants of the microbial mats, even to Chloroflexus species, as the primary producer. In spite of the important ecological role and the phylogenetic diversity in hot springs, the taxonomic study of thermophilic cyanobacteria has made little progress partly because the classical taxonomy of cyanobacteria has been based on morphology. It is urgently necessary to establish the proper classification of thermophilic cyanobacteria. We try to accomplish the taxonomic rearrangement.
Aerobic photosynthetic bacteria newly discovered in the river
A different type of photosynthetic microbial consortia exists in the shallow bottom of the river. The river consortia are mainly composed by green algae, diatoms, and cyanobacteria as phototrophic constituents, but they also commonly include various phylotypes of aerobic photosynthetic bacteria, which are unique aerobes mainly grown by respiration but have bacteriochlorophyll a for photosynthesis even under the aerobic conditions. The reason why these aerobic photosynthetic bacteria are widely distributed in the river consortia is still unknown. To figure out their ecological role in the environments, we are planning to obtain many new isolates further from the bottom in the river and analyze their physiological and genomic properties in detail. Systematic descriptions of newly found species and genus are also intended.
Recent Publications
  1. Nagashima, Sakiko, Akiko Kamimura, Takayuki Shimizu, Sanae Nakamura-Isaki, Eiji Aono, Koji Sakamoto, Natsuko Ichikawa, Hidekazu Nakazawa, Mitsuo Sekine, Shuji Yamazaki, Nobuyuki Fujita, Keizo Shimada, Satoshi Hanada and Kenji VP Nagashima (2012) Complete genome sequence of phototrophic betaproteobacterium Rubrivivax gelatinosus IL144. J Bacteriol 194: 3541-3542
  2. Tsukatani, Yusuke, Nahomi Nakayama, Keizo Shimada, Hiroyuki Mino, Shigeru Itoh, Katsumi Matsuura, Satoshi Hanada and Kenji V. P. Nagashima (2009) Characterization of a blue copper protein, auracyanin, of the filamentous anoxygenic phototrophic bacterium Roseiflexus castenholzii. Arch Biochem Biophys 490:57–62
  3. Yamada, Mitsunori, Hui Zhang, Satoshi Hanada, Kenji V. P. Nagashima, Keizo Shimada and Katsumi Matsuura (2005) Structural and Spectroscopic Properties of a Reaction Center Complex from the Chlorosome-lacking Filamentous Anoxygenic Phototrophic Bacterium Roseiflexus castenholzii. J Bacteriol, 187:1702-1709
  4. Van der Meer, Marcel T.J., Stefan Schouten, Satoshi Hanada, Ellen C. Hopmans, Jaap S. Sinninghe Damsté and David M. Ward (2002) Alkane-1,2-diol-based glycosides and fatty glycosides and wax esters in Roseiflexus castenholzii and hot spring microbial mats. Arch Microbiol, 178:229-237
  5. Hanada, Satoshi, Keizo Shimada and Katsumi Matsuura (2002) Active and energy-dependent rapid formation of cell aggregates of the thermophilic photosynthetic bacterium Chloroflexus aggregans. FEMS Microbiol Lett, 208: 275-279
  6. Suyama Tetsushi, Toru Shigematsu, Yutaka Tokiwa, Takahiro Kanagawa, Kenji V. P. Nagashima and Satoshi Hanada (2002) Photosynthetic apparatus in Roseateles depolymerans 61A is transcriptionally induced by carbon limitation. Appl Environ Microbiol 68:1665-1673
  7. Hanada, Satoshi, Sinichi Takaichi, Katsumi Matsuura and Kazunori Nakamura (2002) Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium which lacks chlorosomes. Int J Syst Evol Microbiol, 52: 187-193
  8. Sugiura, Miwa, Masao Takano, Shin-ichi Kawakami, Katsumi Toda and Satoshi Hanada (2001) Application of a portable spectrophotometer to microbial mat studies: Temperature dependence of the distribution of cyanobacteria and photosynthetic bacteria in hot spring water. Microbes and Environments, 16:255-261
  9. Takaichi, Shinichi, Takashi Maoka, Mitsunori Yamada, Katsumi Matsuura, Yuji Haikawa and Satoshi Hanada (2001) Absence of carotenes and presence of a tertiary methoxy group in a carotenoid form a thermophilic filamentous photosynthetic bacterium Roseiflexus castenholzii, which lacks chlorosomes. Plant Cell Physiol, 42:1355-1362
  10. Suyama, Tetsushi, Toru Shigematsu, Shinichi Takaichi, Yoshinobu Nodasaka, Seizo Fujikawa, Hiroyuki Hosoya, Yutaka Tokiwa, Takahiro Kanagawa, and Satoshi Hanada (1999) Roseateles depolymerans gen. nov., sp. nov., a new bacteriochlorophyll a-containing obligate aerobe belonging to the -subclass of the Proteobacteria. Int. J. Syst. Bacteriol. 49:449-457
  11. Hanada, Satoshi, Toru Shigematsu, Katsutoshi Shibuya, Masahiro Eguchi, Takeshi Hasegawa, Fusako Suda, Yoichi Kamagata, Takahiro Kanagawa and Ryuichiro Kurane (1998) Phylogenetic analysis of trichloroethylene-degrading bacteria newly isolated from the polluted soil with the contaminant. J. Ferment. Bioeng. 86:539-544
  12. Hanada, Satoshi, Yoshie Kawase, Akira Hiraishi, Shinichi Takaichi, Katsumi Matsuura, Keizo Shimada and Kenji V. P. Nagashima (1997) Porphyrobacter tepidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring. Int. J. Syst. Bacteriol. 47:408-413
  13. Hanada, Satoshi, Akira Hiraishi, Keizo Shimada and Katsumi Matsuura (1995) Chloroflexus aggregans sp. nov., a phototrophic filamentous bacterium which forms dense aggregates by active gliding movement. Int. J. Syst. Bacteriol. 45:676-681
  14. Hanada, Satoshi, Akira Hiraishi, Keizo Shimada and Katsumi Matsuura (1995) Isolation of Chloroflexus sp. and related thermophilic photosynthetic bacteria from hot springs using an improved isolation procedure. J. Gen. Appl. Microbiol. 41:119-130
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