最近の研究業績
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- Takahashi, T., Minami, S., Tajima, K., Tsuchiya, Y., Sakai, N., Suga, K., Hisanaga, S., Obayashi, N., Fukuda, M., and Kawahara, H. (2019) Cytoplasmic control of Rab-family small GTPases through BAG6. EMBO Rep. in press
- Demizu, S., Asaka, M., Kawahara, H. and Sasaki, E. (2019) TAS-203, an oral phosphodiesterase 4 inhibitor, suppresses goblet cell hyperplasia and MUC5AC production in rodent models. Eur. J. Pharmacol. in press
- Noguchi, A., Adachi, S., Yokota, N., Hatta, T., Natsume, T., and Kawahara, H. (2018) ZFP36L2 is a cell cycle-regulated CCCH-protein necessary for DNA lesion-induced S-phase arrest. Biol. Open 7, doi: 10.1242/bio.031575
- Kondo, M., Noguchi, A., Matsuura, Y., Shimada, M., Yokota, N., Kawahara, H. (2018) Novel phosphorelay-dependent control of ZFP36L1 protein during the cell cycle. Biochem. Biophys. Res. Comm. 501, 387-393.
- Xuan, X., Matsumoto, S., Endo, S., Fukushima, A., Kawahara, H., Saeki, Y., and Komada, M. (2018) Deubiquitinases USP5 and USP13 are recruited to and regulate heat-induced stress granules by deubiquitinating activities. J. Cell Sci. 131, doi: 10.1242/jcs.210856
- Yamamoto, K., Hayashishita, M., Minami, S., Suzuki, K., Hagiwara, T., Noguchi, A., and Kawahara, H. (2017) Elimination of a signal-sequence uncleaved form of mislocalized HLA protein through BAG6. Sci. Rep. 7, doi:10.1038/s41598-017-14975-9
- Suzuki, R. and Kawahara, H. (2016) UBQLN4 recognizes mislocalized transmembrane domain proteins and targets these to proteasomal degradation. EMBO Rep. 17, 842–857.
- Tanaka, H., Takahashi, T., Xie, Y., Minami, R., Yanagi, Y., Hayashishita, M., Suzuki, R., Yokota, N., Shimada, M., Mizushima, T., Kuwabara, N., Kato, R., and Kawahara, H. (2016) A conserved island of BAG6/Scythe is related to ubiquitin domains and participates in short hydrophobicity recognition. FEBS J. 283, 662–677. DOI: 10.1111/febs.13618
- Takasugi, T., Minegishi, S., Asada, A., Saito, T., Kawahara, H. and Hisanaga, S.-I. (2016) Two degradation pathways of the p35 Cdk5 activation subunit, dependent and independent of ubiquitination. J. Biol. Chem. 291, 4649-4657. doi: 10.1074/jbc.M115.692871
- Yamaki, Y., Kagawa, H., Hatta, T., Natsume, T., and Kawahara, H. (2016) The C-terminal cytoplasmic tail of hedgehog receptor Patched1 is a platform for E3 ubiquitin ligase complexes. Mol. Cell. Biochem. 414: 1-12. DOI: 10.1007/s11010-015-2643-4
- Kuwabara, N., Minami, R., Yokota, N., Matsumoto, H., Senda, T., Kawahara, H., and Kato, R. (2015) Structure of a BAG6–Ubl4a complex reveals a novel binding interface that functions in tail-anchored protein biogenesis. J. Biol. Chem. 290, 9387-9398.
- Kawahara, H., Minami, R. and Yokota, N. (2013) JB Review: BAG6 / BAT3: Emerging roles in quality control for nascent polypeptides. J. Biochem. 153, 147-160.
- Kagawa, H.,, Shino, Y., Kobayashi, D., Demizu, S., Shimada. M., Ariga, H. and Kawahara, H. (2011) A novel signaling pathway mediated by the nuclear targeting of C-terminal fragments of mammalian Patched 1. PLoS ONE 6: e18638. doi:10.1371/journal.pone.0018638
- Sato, K., Minegishi, S., Takano, J., Plattner, F., Saito, T., Asada, A., Kawahara, H., Iwata, N., Saido, T.C., and Hisanaga, S. (2011) Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25. J. Neurochem. 117:504-515.
- Yokota, N., Kataoka, Y., Hashii, N., Kawasaki, N., and Sawada, H. (2011) Sperm-specific C-terminal processing of the proteasome PSMA1/α6 subunit. Biochem. Biophys. Res. Commun. 410:809-815
- Minami, R., Hayakawa, A., Kagawa, H., Yanagi, Y., Yokosawa, H. and Kawahara, H. (2010) BAG-6 is essential for selective elimination of defective proteasomal substrates. J. Cell Biol. 190: 637-650.
- Ojima, K., Y Kawabata, Y., Nakao, H., Nakao, K., Doi, N., Kitamura, F., Ono, Y., Hata, S., Suzuki, H., Kawahara, H., Labeit, S., Toyama-Sorimachi, N., Suzuki, K., Maeda, T., Abe, K., Aiba, A., and Sorimachi, H. (2010) Role of dynamic distribution of muscle-specific calpain in physical-stress adaptation and muscular dystrophy in mice. J. Clinc. Invest. 120: 2672−2683.
- Endo, R., Saito, T., Asada, A., Kawahara, H. and Hisanaga, S.-I. (2009) Commitment of 1-methyl-4-phenylpyrinidinium ion-induced neuronal cell death by proteasome-mediated degradation of p35 Cyclin-dependent kinase 5 activator. J. Biol. Chem. 284, 26029-26039.
- Minami, R., Shimada, M., Yokosawa, H. and Kawahara, H. (2007) Scythe regulates apoptosis through modulating ubiquitin-mediated proteolysis of XEF1AO. Biochem. J. (London) 405: 495-501.
- Hamazaki, J., Sasaki, K., Kawahara, H., Hisanaga, S., Tanaka, K., and Murata, S. (2007) Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development. Mol. Cell. Biol. 27: 5529-6638.
- Tayama, Y., Kawahara, H., Minami, R., Shimada, M., and Yokosawa, H. (2007) Association of Rpn10 with high molecular weight complex is enhanced during retinoic acid-induced differentiation of neuroblastoma cells. Mol. Cell. Biochem.
- Shimada, M., Kanematsu, K., Tanaka, K., Yokosawa, H. and Kawahara, H. (2006) Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans. Mol. Biol. Cell 17: 5356-5371.
- Shimada, M., Yokosawa, H., and Kawahara, H. (2006) OMA-1 is a P granules-associated protein that is required for germline specification in C. elegans. Genes Cells 11, 383-396.
- Hata, S., Koyama, S., Kawahara, H., Doi, N., Maeda, T., Toyama-Sorimachi, N., Abe, K., Suzuki, K., and Sorimachi, H. (2006) Stomach-specific calpain localizes in mucus cells and proteolyses the β-subunit of coatomer complex, β-COP. J. Biol. Chem. 281, 11214-11224.
- Kikukawa, Y., Minami, R., Shimada, M., Kobayashi, M., Tanaka, K., Yokosawa, H., and Kawahara, H. (2005) Unique proteasome subunit Xrpn10c is a specific receptor for the antiapoptotic ubiquitin-like protein Scythe. FEBS J. 272, 6373−6386.
- Saeki, Y., Isono, E., Shimada, M., Kawahara, H., Yokosawa, H., and Toh-e, A. (2005) Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically-encodable tandem ubiquitin. Methods Enzymol. 399, 64-74.
- Kinouchi, T., Ishiura, S., Mabuchi, Y., Urakami-Manaka, Y., Nishio, H., Nishiuchi, Y., Tsunemi, M., Takada, K., Watanabe, M., Ikeda, M., Matsui, H., Tomioka, S., Kawahara, H., Hamamoto, T., Suzuki, K., Kagawa, Y. (2004) Mammalian D-aspartyl endopeptidase: a scavenger for noxious racemized proteins in aging. Biochem. Biophys. Res. Comm. 314, 730-736.
- Tsukamoto, S., Yamashita, K., Tane, K., Kizu, R., Ohta, T., Matsunaga, S., Fusetani, N., Kawahara, H., and Yokosawa, H. (2004) Girolline, an antitumor compound isolated from a sponge, induces G2/M cell cycle arrest and accumulation of polyubiquitinated p53. Biol. Pharm. Bull. 27, 699-701.
- Saeki, Y., Isono, E., Oguchi, T., Shimada, M., Sone, T., Kawahara, H., Yokosawa, H., and Toh-e, A. (2004) Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division. Genes Genet. Syst. 79, 77-86.
- Sakata, E., Yamaguchi, Y., Kurimoto, E., Kikuchi, J., Yokoyama, S., Yamada, S., Kawahara, H., Yokosawa, H., Hattori, N., Mizuno, Y., Tanaka, K. and Kato. K. (2003) Parkin binds the Rpn10 subunit of the 26S proteasome with the ubiquitin-like domain. EMBO Rep. 4, 301-306.
- Sato, N., Kawahara, H., Toh-e, A. and Maeda, T. (2003) Phosphorelay-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system. Mol. Cell. Biol. 23, 6662-6671.
- Kawai, N., Shimada, M., Kawahara, H., Satoh, N. and Yokosawa, H. (2003) Regulation of ascidian Rel by its alternative splice variant. Eur. J. Biochem. 270, 4459-4468.
- Shimada, M., Kawahara, H., and Doi, H. (2002) Novel family of CCCH type Zinc-finger proteins; MOE-1, -2 , and -3, participate in C. elegans oocyte maturation. Genes Cells 7, 933-947.
- Kikukawa, Y., Shimada, M., Suzuki, N., Tanaka, K., Yokosawa, H., and Kawahara, H. (2002) The 26S proteasome rpn10 gene encoding splicing isoforms: evolutional conservation of the genomic organization in vertebrates. Biol. Chem. 383, 1257-1261.
- Takahashi, N., Sasagawa, N., Usuki, F., Kino, Y., Kawahara, H., Sorimachi, H., Maeda, T., Suzuki, K., and Ishiura, S. (2001) Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. J. Biochem. 130, 581-587.
- Umeda, T., Kouchi, Z., Kawahara, H., Tomioka, S., Sasagawa, N.,Maeda, T., Sorimachi, H., Ishiura, S., and Suzuki, K. (2001) Limited proteolysis of filamin is catalyzed by caspase-3 in U937 and Jurkat cells. J. Biochem. 130, 535-542.
- Hata, S., Nishi, K., Kawamoto, T., Lee, H.J., Kawahara, H., Maeda, T., Sorimachi, H., Suzuki, K. (2001) Both the conserved and the unique gene structure of stomach-specific calpains reveal processes of calpain gene evolution. J. Mol. Evol. 53, 191-203.
- Kawahara, H., Philipova, R., Yokosawa, H., Patel, R., Tanaka, K. and Whitaker, M. (2000) Inhibiting proteasome activity causes over-replication of DNA and blocks entry into mitosis in sea urchin embryos. J. Cell Sci. 113, 2659-2670.
- Kawahara, H., Kasahara, M., Nishiyama, A., Ohsumi, K., Goto, T., Kishimoto, T., Saeki, Y., Yokosawa, H., Shimbara, N., Mutrata, S., Chiba, T., Suzuki, K. and Tanaka, K. (2000) Developmentally regulated alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes. EMBO J. 19, 4144-4153.
- Murata, S., Kawahara, H., Tohma, S., Kasahara, K., Tanaka, K. and Chiba, T. (1999) Growth retardation of mice lacking the proteasome activator PA28 γ. J. Biol. Chem. 274, 38211-38215.
- 川原裕之 (2015) 特集「ユビキチンシステムの制御と疾患 –その理解と回復に向けて-」「ユビキチンによるタンパク質品質管理と疾患」The World of UBQUITIN、第3巻 p.60-64.
- 川原裕之, 南亮介 (2011)「BAG6を介したタンパク質品質管理の新機構」実験医学、第29巻、7月増刊号, 羊土社
- 嶋田益弥、川原裕之(2010)「卵成熟制御におけるCCCH型zinc-finger蛋白質の機能」「細胞周期フロンティア」共立出版
- 川原裕之, 嶋田益弥(2007)「生殖細胞の分化運命決定の分子機構」バイオとナノの融合(I)新生命科学の基礎(分担執筆)、pp.183-191,北海道大学出版会.
- 川原裕之, 嶋田益弥, 南亮介, 横沢英良 (2006)「ユビキチンレセプターの構造と機能」蛋白質核酸酵素, 増刊号pp.1245-1250,共立出版.
- 佐伯泰, 川原裕之, 横沢英良 (2003)「ユビキチンと相互作用するドメイン構造」実験医学、21, 340-345, 羊土社
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